› 3cz1

X-ray diffraction
1.5Å resolution

Dimeric crystal structure of a pheromone binding protein from Apis mellifera in complex with the n-butyl benzene sulfonamide at pH 7.0

Released:
DOI: 10.2210/pdb3cz1/pdb
PDB entry 3cz1 coloured by chain, front view.
PDB entry 3cz1 coloured by chain, side view.
PDB entry 3cz1 coloured by chain, top view.
Source organism: Apis mellifera
Primary publication:
Queen bee pheromone binding protein pH-induced domain swapping favors pheromone release.
Pesenti ME, Spinelli S, Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
J Mol Biol 390 981-90 (2009)
PMID: 19481550

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193638 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pheromone-binding protein ASP1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 13.19 KDa
Source organism: Apis mellifera
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9U9J6 (Residues: 26-144; Coverage: 100%)
Sequence domains: PBP/GOBP family
Structure domains: Pheromone/general odorant binding protein domain

Ligands and Environments

4 bound ligands:
Ligand NBB 3 x NBB
Ligand GOL 3 x GOL
Ligand MG 1 x MG
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 35.413Å b: 75.652Å c: 84.101Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.152 0.198
Expression system: Komagataella pastoris

Quick links

Citations

3 review citations

A look inside odorant-binding proteins in insect chemoreception.
Brito et al. (2016)
2 more

1 mention without citation

Mapping the Anopheles gambiae odorant binding protein 1 (AgamOBP1) using modeling techniques, site directed mutagenesis, circular dichroism and ligand binding assays.
Rusconi et al. (2012)