› 3czm

X-ray diffraction
2.3Å resolution

T. Gondii bradyzoite-specific LDH (LDH2) in complex with NAD and OXQ

Released:
DOI: 10.2210/pdb3czm/pdb
PDB entry 3czm coloured by chain, front view.
PDB entry 3czm coloured by chain, side view.
PDB entry 3czm coloured by chain, top view.
Source organism: Toxoplasma gondii
Entry authors: Wilson DK, Susko MS

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 35.38 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27797 (Residues: 1-326; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand OXQ 2 x OXQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P321
Unit cell:
a: 140.949Å b: 140.949Å c: 75.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.166 0.223
Expression system: Escherichia coli

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