› 3d11

X-ray diffraction
2.31Å resolution

Crystal Structures of the Nipah G Attachment Glycoprotein

Released:
DOI: 10.2210/pdb3d11/pdb
PDB entry 3d11 coloured by chain, front view.
PDB entry 3d11 coloured by chain, side view.
PDB entry 3d11 coloured by chain, top view.
Source organism: Nipah henipavirus
Primary publication:
Host cell recognition by the henipaviruses: crystal structures of the Nipah G attachment glycoprotein and its complex with ephrin-B3.
Xu K, Rajashankar KR, Chan YP, Himanen JP, Broder CC, Nikolov DB
Proc Natl Acad Sci U S A 105 9953-8 (2008)
PMID: 18632560

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glycoprotein G Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 47.97 KDa
Source organism: Nipah henipavirus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9IH62 (Residues: 176-602; Coverage: 71%)
Gene name: G
Sequence domains: Haemagglutinin-neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand IOD 19 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P213
Unit cell:
a: 130.161Å b: 130.161Å c: 130.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.257
Expression system: Spodoptera frugiperda

Quick links

Citations

33 review citations

Viral entry mechanisms: the increasing diversity of paramyxovirus entry.
Smith et al. (2009)
32 more

32 mentions without citation

Analysis of the conserved protective epitopes of hemagglutinin on influenza A viruses.
Jiao et al. (2023)
31 more