› 3d5k

X-ray diffraction
2.4Å resolution

Crystal structure of the OprM channel in a non-symmetrical space group

Released:
DOI: 10.2210/pdb3d5k/pdb
PDB entry 3d5k coloured by chain, front view.
PDB entry 3d5k coloured by chain, side view.
PDB entry 3d5k coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural and dynamical insights into the opening mechanism of P. aeruginosa OprM channel.
Phan G, Benabdelhak H, Lascombe MB, Benas P, Rety S, Picard M, Ducruix A, Etchebest C, Broutin I
Structure 18 507-17 (2010)
PMID: 20399187

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein OprM Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 474 amino acids
Theoretical weight: 51.78 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51487 (Residues: 18-485; Coverage: 100%)
Gene names: PA0427, oprK, oprM
Sequence domains: Outer membrane efflux protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 9 x NA
Ligand CL 1 x CL
1 modified residue:
Ligand SCY 3 x SCY

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 73.9Å b: 70.2Å c: 344.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.236 0.293
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Interaction of antibacterial compounds with RND efflux pumps in Pseudomonas aeruginosa.
Dreier et al. (2015)
7 more

12 mentions without citation

Carbapenems: past, present, and future.
Papp-Wallace et al. (2011)
11 more