› 3d6t

X-ray diffraction
2.43Å resolution

Structure of the ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2 reveals a dimeric GTPase

Released:
DOI: 10.2210/pdb3d6t/pdb
PDB entry 3d6t coloured by chain, front view.
PDB entry 3d6t coloured by chain, side view.
PDB entry 3d6t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2 reveals a dimeric GTPase.
Deng J, Lewis PA, Greggio E, Sluch E, Beilina A, Cookson MR
Proc Natl Acad Sci U S A 105 1499-504 (2008)
PMID: 18230735

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-177793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine-rich repeat serine/threonine-protein kinase 2 Chain: B
Molecule details ›
Chain: B
Length: 171 amino acids
Theoretical weight: 19.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5S007 (Residues: 1335-1505; Coverage: 7%)
Gene names: LRRK2, PARK8
Sequence domains: Ras of Complex, Roc, domain of DAPkinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 69.919Å b: 53.167Å c: 42.736Å
α: 90° β: 112.76° γ: 90°
R-values:
R R work R free
0.216 0.211 0.307
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

56 review citations

Mitochondrial dysfunction in Parkinson's disease: molecular mechanisms and pathophysiological consequences.
Exner et al. (2012)
55 more

2 mentions without citation

Parkinson disease-associated mutation R1441H in LRRK2 prolongs the "active state" of its GTPase domain.
Liao et al. (2014)
1 more