› 3d73

X-ray diffraction
2.03Å resolution

Crystal structure of a pheromone binding protein mutant D35A, from Apis mellifera, at pH 7.0

Released:
DOI: 10.2210/pdb3d73/pdb
PDB entry 3d73 coloured by chain, front view.
PDB entry 3d73 coloured by chain, side view.
PDB entry 3d73 coloured by chain, top view.
Source organism: Apis mellifera
Primary publication:
Queen bee pheromone binding protein pH-induced domain swapping favors pheromone release.
Pesenti ME, Spinelli S, Bezirard V, Briand L, Pernollet JC, Campanacci V, Tegoni M, Cambillau C
J Mol Biol 390 981-90 (2009)
PMID: 19481550

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pheromone-binding protein ASP1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 13.15 KDa
Source organism: Apis mellifera
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9U9J6 (Residues: 26-144; Coverage: 100%)
Sequence domains: PBP/GOBP family
Structure domains: Pheromone/general odorant binding protein domain

Ligands and Environments

1 bound ligand:
Ligand NBB 3 x NBB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21212
Unit cell:
a: 61.873Å b: 60.859Å c: 56.682Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.224
Expression system: Komagataella pastoris

Quick links

Citations

3 review citations

A look inside odorant-binding proteins in insect chemoreception.
Brito et al. (2016)
2 more

1 mention without citation

A subset of chemosensory genes differs between two populations of a specialized leaf beetle after host plant shift.
Wang et al. (2018)