› 3dcg

X-ray diffraction
2.4Å resolution

Crystal Structure of the HIV Vif BC-box in Complex with Human ElonginB and ElonginC

Released:
DOI: 10.2210/pdb3dcg/pdb
PDB entry 3dcg coloured by chain, front view.
PDB entry 3dcg coloured by chain, side view.
PDB entry 3dcg coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into the human immunodeficiency virus Vif SOCS box and its role in human E3 ubiquitin ligase assembly.
Stanley BJ, Ehrlich ES, Short L, Yu Y, Xiao Z, Yu XF, Xiong Y
J Virol 82 8656-63 (2008)
PMID: 18562529

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-146245 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Elongin-B Chains: A, C
Molecule details ›
Chains: A, C
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chains: B, D
Molecule details ›
Chains: B, D
Length: 97 amino acids
Theoretical weight: 10.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
p7 Chains: E, F
Molecule details ›
Chains: E, F
Length: 39 amino acids
Theoretical weight: 4.45 KDa
Source organism: Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE)
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P12504 (Residues: 139-176; Coverage: 20%)
Gene name: vif

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 55.514Å b: 66.913Å c: 122.642Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.232
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

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Sarikas et al. (2011)
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19 mentions without citation

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