› 3ddt

X-ray diffraction
1.9Å resolution

Crystal structure of the B2 box from MuRF1 in dimeric state

Released:
DOI: 10.2210/pdb3ddt/pdb
PDB entry 3ddt coloured by chain, front view.
PDB entry 3ddt coloured by chain, side view.
PDB entry 3ddt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of B-Box 2 from MuRF1: identification of a novel self-association pattern in a RING-like fold.
Mrosek M, Meier S, Ucurum-Fotiadis Z, von Castelmur E, Hedbom E, Lustig A, Grzesiek S, Labeit D, Labeit S, Mayans O
Biochemistry 47 10722-30 (2008)
PMID: 18795805

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase TRIM63 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 48 amino acids
Theoretical weight: 5.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q969Q1 (Residues: 117-161; Coverage: 13%)
Gene names: IRF, MURF1, RNF28, SMRZ, TRIM63
Sequence domains: B-box zinc finger
Structure domains: Classic Zinc Finger

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA, ESRF BEAMLINE ID23-1
Spacegroup: P6522
Unit cell:
a: 76.22Å b: 76.22Å c: 146.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.252
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1.
Bodine et al. (2014)
5 more

7 mentions without citation

Conserved structural and functional aspects of the tripartite motif gene family point towards therapeutic applications in multiple diseases.
Gushchina et al. (2018)
6 more