› 3deo

X-ray diffraction
1.5Å resolution

Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43

Released:
DOI: 10.2210/pdb3deo/pdb
PDB entry 3deo coloured by chain, front view.
PDB entry 3deo coloured by chain, side view.
PDB entry 3deo coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43.
Stengel KF, Holdermann I, Cain P, Robinson C, Wild K, Sinning I
Science 321 253-6 (2008)
PMID: 18621669

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle 43 kDa protein, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.04 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O22265 (Residues: 85-267; Coverage: 49%)
Gene names: At2g47450, CAO, CPSRP43, T30B22.25
Sequence domains: Ankyrin repeat
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 51.146Å b: 52.794Å c: 62.849Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.211
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
13 more

6 mentions without citation

Molecular mechanism of SRP-dependent light-harvesting protein transport to the thylakoid membrane in plants.
Ziehe et al. (2018)
5 more