› 3dgr

X-ray diffraction
2.1Å resolution

Crystal structure of human NAMPT complexed with ADP analogue

Released:
DOI: 10.2210/pdb3dgr/pdb
PDB entry 3dgr coloured by chain, front view.
PDB entry 3dgr coloured by chain, side view.
PDB entry 3dgr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT.
Burgos ES, Ho MC, Almo SC, Schramm VL
Proc Natl Acad Sci U S A 106 13748-53 (2009)
PMID: 19666527

Function and Biology Details

Reaction catalysed: 
Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 484 amino acids
Theoretical weight: 54.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43490 (Residues: 1-484; Coverage: 99%)
Gene names: NAMPT, PBEF, PBEF1
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand A12 2 x A12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 61.29Å b: 107.229Å c: 82.661Å
α: 90° β: 96.41° γ: 90°
R-values:
R R work R free
0.182 0.18 0.225
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Nicotinamide phosphoribosyltransferase in malignancy: a review.
Shackelford et al. (2013)
11 more

5 mentions without citation

A review on graphene-based nanocomposites for electrochemical and fluorescent biosensors.
Krishnan et al. (2019)
4 more