› 3dgt

X-ray diffraction
1.5Å resolution

The 1.5 A crystal structure of endo-1,3-beta-glucanase from Streptomyces sioyaensis

Released:
DOI: 10.2210/pdb3dgt/pdb
PDB entry 3dgt coloured by chain, front view.
PDB entry 3dgt coloured by chain, side view.
PDB entry 3dgt coloured by chain, top view.
Source organism: Streptomyces sioyaensis
Primary publication:
The 1.5 A structure of endo-1,3-beta-glucanase from Streptomyces sioyaensis: evolution of the active-site structure for 1,3-beta-glucan-binding specificity and hydrolysis.
Hong TY, Hsiao YY, Meng M, Li TT
Acta Crystallogr D Biol Crystallogr 64 964-70 (2008)
PMID: 18703845

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192224 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,3-beta-glucanase Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 29.38 KDa
Source organism: Streptomyces sioyaensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L816 (Residues: 49-321; Coverage: 63%)
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 39.509Å b: 75.967Å c: 79.663Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.198
Expression system: Escherichia coli

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Citations

2 citation in other articles

Mode of operation and low-resolution structure of a multi-domain and hyperthermophilic endo-β-1,3-glucanase from Thermotoga petrophila.
Cota et al. (2011)
1 more

5 mentions without citation

Advances of Artificial Intelligence in Anti-Cancer Drug Design: A Review of the Past Decade.
Wang et al. (2023)
4 more