› 3dhu

X-ray diffraction
2Å resolution

Crystal structure of an alpha-amylase from Lactobacillus plantarum

Released:
DOI: 10.2210/pdb3dhu/pdb
PDB entry 3dhu coloured by chain, front view.
PDB entry 3dhu coloured by chain, side view.
PDB entry 3dhu coloured by chain, top view.
Source organism: Lactiplantibacillus plantarum
Entry authors: Bonanno JB, Dickey M, Bain KT, Iizuka M, Ozyurt S, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 449 amino acids
Theoretical weight: 51.11 KDa
Source organism: Lactiplantibacillus plantarum
Expression system: Escherichia coli
UniProt:
  • Canonical: F9USZ1 (Residues: 3-440; Coverage: 100%)
Gene names: lp_0179, malS
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 202.279Å b: 63.773Å c: 155.677Å
α: 90° β: 102.93° γ: 90°
R-values:
R R work R free
0.203 0.2 0.251
Expression system: Escherichia coli

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