› 3dls

X-ray diffraction
2.3Å resolution

Crystal structure of human PAS kinase bound to ADP

Released:
DOI: 10.2210/pdb3dls/pdb
PDB entry 3dls coloured by chain, front view.
PDB entry 3dls coloured by chain, side view.
PDB entry 3dls coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural bases of PAS domain-regulated kinase (PASK) activation in the absence of activation loop phosphorylation.
Kikani CK, Antonysamy SA, Bonanno JB, Romero R, Zhang FF, Russell M, Gheyi T, Iizuka M, Emtage S, Sauder JM, Turk BE, Burley SK, Rutter J
J Biol Chem 285 41034-43 (2010)
PMID: 20943661

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-188719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAS domain-containing serine/threonine-protein kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 335 amino acids
Theoretical weight: 37.88 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q96RG2 (Residues: 977-1300; Coverage: 25%)
Gene names: KIAA0135, PASK
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 19 x MG
Ligand ADP 6 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 85.778Å b: 85.84Å c: 94.154Å
α: 77.28° β: 77.5° γ: 60.09°
R-values:
R R work R free
0.244 0.241 0.297
Expression system: unidentified baculovirus

Quick links

Citations

5 review citations

PAS kinase: a nutrient sensing regulator of glucose homeostasis.
DeMille et al. (2013)
4 more

10 mentions without citation

Comparison of structure-based and threading-based approaches to protein functional annotation.
Brylinski et al. (2010)
9 more