› 3dm9

X-ray diffraction
2.2Å resolution

Structures and Conformations in Solution of the Signal Recognition Particle Receptor from the archaeon Pyrococcus furiosus

Released:
DOI: 10.2210/pdb3dm9/pdb
PDB entry 3dm9 coloured by chain, front view.
PDB entry 3dm9 coloured by chain, side view.
PDB entry 3dm9 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structures of the signal recognition particle receptor from the archaeon Pyrococcus furiosus: implications for the targeting step at the membrane.
Egea PF, Tsuruta H, de Leon GP, Napetschnig J, Walter P, Stroud RM
PLoS One 3 e3619 (2008)
PMID: 18978942

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186364 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle receptor FtsY Chain: B
Molecule details ›
Chain: B
Length: 328 amino acids
Theoretical weight: 36.67 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U051 (Residues: 1-322; Coverage: 100%)
Gene names: PF1766, ftsY
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 4 x PO4
Ligand MPD 1 x MPD
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P622
Unit cell:
a: 144.6Å b: 144.6Å c: 70.413Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.203 0.239
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Archaea signal recognition particle shows the way.
Zwieb et al. (2010)
1 more

1 mention without citation

Structures of the signal recognition particle receptor from the archaeon Pyrococcus furiosus: implications for the targeting step at the membrane.
Egea et al. (2008)