› 3dnm

X-ray diffraction
2.8Å resolution

Crystal Structure Hormone-Sensitive Lipase from a Metagenome Library

Released:
DOI: 10.2210/pdb3dnm/pdb
PDB entry 3dnm coloured by chain, front view.
PDB entry 3dnm coloured by chain, side view.
PDB entry 3dnm coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Structural and functional analysis of a novel hormone-sensitive lipase from a metagenome library.
Nam KH, Kim MY, Kim SJ, Priyadarshi A, Kwon ST, Koo BS, Yoon SH, Hwang KY
Proteins 74 1036-40 (2009)
PMID: 19089974

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-170931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 336 amino acids
Theoretical weight: 36.01 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0GMU1 (Residues: 1-309; Coverage: 100%)
Gene name: estE7
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand BME 4 x BME
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: I212121
Unit cell:
a: 117.321Å b: 126.828Å c: 233.393Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.282
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
1 more

5 mentions without citation

Structural basis for dimerization and catalysis of a novel esterase from the GTSAG motif subfamily of the bacterial hormone-sensitive lipase family.
Li et al. (2014)
4 more