› 3dnu

X-ray diffraction
1.54Å resolution

structure of MDT protein

Released:
DOI: 10.2210/pdb3dnu/pdb
PDB entry 3dnu coloured by chain, front view.
PDB entry 3dnu coloured by chain, side view.
PDB entry 3dnu coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB.
Schumacher MA, Piro KM, Xu W, Hansen S, Lewis K, Brennan RG
Science 323 396-401 (2009)
PMID: 19150849

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase toxin HipA Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 49.87 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P23874 (Residues: 1-440; Coverage: 100%)
Gene names: JW1500, b1507, hipA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand PO4 3 x PO4
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 48.66Å b: 85.81Å c: 49.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.232
Expression system: Escherichia coli

Quick links

Citations

72 review citations

Persister cells.
Lewis K. (2010)
71 more

3 mentions without citation

Wake me when it's over - Bacterial toxin-antitoxin proteins and induced dormancy.
Coussens et al. (2016)
2 more