› 3do7

X-ray diffraction
3.05Å resolution

X-ray structure of a NF-kB p52/RelB/DNA complex

Released:
DOI: 10.2210/pdb3do7/pdb
PDB entry 3do7 coloured by chain, front view.
PDB entry 3do7 coloured by chain, side view.
PDB entry 3do7 coloured by chain, top view.
Source organisms:
Entry authors: Fusco A, Huang DB, Miller D, Vu D, Ghosh G

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-116182 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Transcription factor RelB Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04863 (Residues: 88-383; Coverage: 53%)
Gene name: Relb
Sequence domains:
Structure domains:
Nuclear factor NF-kappa-B p52 subunit Chain: B
Molecule details ›
Chain: B
Length: 293 amino acids
Theoretical weight: 33.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00653 (Residues: 37-329; Coverage: 33%)
Gene names: LYT10, NFKB2
Sequence domains:
Structure domains:
5'-D(*DCP*DGP*DGP*DGP*DAP*DAP*DTP*DTP*DCP*DCP*DC)-3' Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 11 nucleotides
Theoretical weight: 3.33 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 77.64Å b: 124.13Å c: 189.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.273
Expression system: Escherichia coli BL21(DE3)

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