› 3dpc

X-ray diffraction
2.3Å resolution

Structure of E.coli Alkaline Phosphatase Mutant in Complex with a Phosphorylated Peptide

Released:
DOI: 10.2210/pdb3dpc/pdb
PDB entry 3dpc coloured by chain, front view.
PDB entry 3dpc coloured by chain, side view.
PDB entry 3dpc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Development of a universal phosphorylated peptide-binding protein for simultaneous assay of kinases.
Li W, Bi L, Wang W, Li Y, Zhou Y, Wei H, Jiang T, Bai L, Chen Y, Zhang Z, Yuan X, Xiao J, Zhang XE
Biosens Bioelectron 24 2871-7 (2009)
PMID: 19349157

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132774 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 47.95 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00634 (Residues: 23-471; Coverage: 100%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A
Phosphorylated Peptide Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.18 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand TRS 1 x TRS
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P212121
Unit cell:
a: 64.862Å b: 107.74Å c: 148.389Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.271
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

High-resolution analysis of Zn(2+) coordination in the alkaline phosphatase superfamily by EXAFS and x-ray crystallography.
Bobyr et al. (2012)
1 more

11 mentions without citation

Photonic crystal based biosensors: Emerging inverse opals for biomarker detection.
Fathi et al. (2021)
10 more