› 3dqw

X-ray diffraction
2.02Å resolution

c-Src kinase domain Thr338Ile mutant in complex with ATPgS

Released:
DOI: 10.2210/pdb3dqw/pdb
PDB entry 3dqw coloured by chain, front view.
PDB entry 3dqw coloured by chain, side view.
PDB entry 3dqw coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Activation of tyrosine kinases by mutation of the gatekeeper threonine.
Azam M, Seeliger MA, Gray NS, Kuriyan J, Daley GQ
Nat Struct Mol Biol 15 1109-18 (2008)
PMID: 18794843

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 286 amino acids
Theoretical weight: 32.82 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00523 (Residues: 251-533; Coverage: 53%)
Gene name: SRC
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AGS 4 x AGS
Ligand MG 4 x MG
1 modified residue:
Ligand PTR 4 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 82.42Å b: 103.65Å c: 83.69Å
α: 90° β: 103.94° γ: 90°
R-values:
R R work R free
0.207 0.205 0.246
Expression system: Escherichia coli

Quick links

Citations

71 review citations

A view on drug resistance in cancer.
Vasan et al. (2019)
70 more

19 mentions without citation

The Src module: an ancient scaffold in the evolution of cytoplasmic tyrosine kinases.
Shah et al. (2018)
18 more