› 3dsl

X-ray diffraction
2.7Å resolution

The Three-dimensional Structure of Bothropasin, the Main Hemorrhagic Factor from Bothrops jararaca venom.

Released:
DOI: 10.2210/pdb3dsl/pdb
PDB entry 3dsl coloured by chain, front view.
PDB entry 3dsl coloured by chain, side view.
PDB entry 3dsl coloured by chain, top view.
Source organism: Bothrops jararaca
Primary publication:
The three-dimensional structure of bothropasin, the main hemorrhagic factor from Bothrops jararaca venom: insights for a new classification of snake venom metalloprotease subgroups.
Muniz JR, Ambrosio AL, Selistre-de-Araujo HS, Cominetti MR, Moura-da-Silva AM, Oliva G, Garratt RC, Souza DH
Toxicon 52 807-16 (2008)
PMID: 18831982

Function and Biology Details

Reaction catalysed: 
Cleavage of 5-His-|-Leu-6, 10-His-|-Leu-11, 14-Ala-|-Leu-15, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 in insulin B chain.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zinc metalloproteinase-disintegrin-like bothropasin Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 46.69 KDa
Source organism: Bothrops jararaca
UniProt:
  • Canonical: O93523 (Residues: 192-610; Coverage: 71%)
Sequence domains:
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

5 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 6 x CA
Ligand ZN 2 x ZN
Ligand FLE 2 x FLE
Ligand TRP 2 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P212121
Unit cell:
a: 73.757Å b: 100.271Å c: 133.237Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.21 0.294

Quick links

Citations

5 review citations

Key events in microvascular damage induced by snake venom hemorrhagic metalloproteinases.
Escalante et al. (2011)
4 more

9 mentions without citation

ADAM and ADAMTS Family Proteins and Snake Venom Metalloproteinases: A Structural Overview.
Takeda S. (2016)
8 more