› 3dst

X-ray diffraction
1.9Å resolution

Crystal structure of RabGGTase(DELTA LRR; DELTA IG)in complex with geranylgeranyl pyrophosphate

Released:
DOI: 10.2210/pdb3dst/pdb
PDB entry 3dst coloured by chain, front view.
PDB entry 3dst coloured by chain, side view.
PDB entry 3dst coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structures of RabGGTase-substrate/product complexes provide insights into the evolution of protein prenylation.
Guo Z, Wu YW, Das D, Delon C, Cramer J, Yu S, Thuns S, Lupilova N, Waldmann H, Brunsveld L, Goody RS, Alexandrov K, Blankenfeldt W
EMBO J 27 2444-56 (2008)
PMID: 18756270

Function and Biology Details

Reaction catalysed: 
Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170665 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Geranylgeranyl transferase type-2 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 38.44 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08602 (Residues: 1-237, 353-441; Coverage: 58%)
Gene names: Ggta, Rabggta
Sequence domains: Protein prenyltransferase alpha subunit repeat
Structure domains: Protein prenylyltransferase
Geranylgeranyl transferase type-2 subunit beta Chain: B
Molecule details ›
Chain: B
Length: 331 amino acids
Theoretical weight: 36.89 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08603 (Residues: 1-331; Coverage: 100%)
Gene names: Ggtb, Rabggtb
Sequence domains: Prenyltransferase and squalene oxidase repeat
Structure domains: Glycosyltransferase

Ligands and Environments

3 bound ligands:
Ligand GRG 1 x GRG
Ligand ZN 1 x ZN
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 67.264Å b: 90.618Å c: 114.478Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.22
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Protein prenylation: enzymes, therapeutics, and biotechnology applications.
Palsuledesai et al. (2015)
13 more

9 mentions without citation

Targeting Small GTPases and Their Prenylation in Diabetes Mellitus.
Gendaszewska-Darmach et al. (2021)
8 more