› 3dsz

X-ray diffraction
2Å resolution

Engineered human lipocalin 2 in complex with Y-DTPA

Released:
DOI: 10.2210/pdb3dsz/pdb
PDB entry 3dsz coloured by chain, front view.
PDB entry 3dsz coloured by chain, side view.
PDB entry 3dsz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2.
Kim HJ, Eichinger A, Skerra A
J Am Chem Soc 131 3565-76 (2009)
PMID: 19227970

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil gelatinase-associated lipocalin Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 21.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P80188 (Residues: 21-198; Coverage: 100%)
Gene names: HNL, LCN2, NGAL
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand LIZ 2 x LIZ
Ligand YT3 2 x YT3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41212
Unit cell:
a: 82.35Å b: 82.35Å c: 115.128Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.23
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Engineered protein scaffolds as next-generation antibody therapeutics.
Gebauer et al. (2009)
4 more

4 mentions without citation

Structural insights for engineering binding proteins based on non-antibody scaffolds.
Gilbreth et al. (2012)
3 more