› 3dtp

Electron Microscopy
20Å resolution

Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP

Released:
DOI: 10.2210/pdb3dtp/pdb
PDB entry 3dtp coloured by chain, front view.
PDB entry 3dtp coloured by chain, side view.
PDB entry 3dtp coloured by chain, top view.
Source organisms:
Primary publication:
Three-dimensional reconstruction of tarantula myosin filaments suggests how phosphorylation may regulate myosin activity.
Alamo L, Wriggers W, Pinto A, Bártoli F, Salazar L, Zhao FQ, Craig R, Padrón R
J Mol Biol 384 780-97 (2008)
PMID: 18951904
Related structures: EMD-1950

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 216-mer (preferred)
PDBe Complex ID:
PDB-CPX-110152 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Myosin-11; Myosin-7 Chain: A
Molecule details ›
Chain: A
Length: 971 amino acids
Theoretical weight: 111.85 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10587 (Residues: 2-852; Coverage: 43%)
  • Canonical: P12883 (Residues: 842-961; Coverage: 6%)
Gene names: MYH11, MYH7, MYHCB
Sequence domains:
Myosin-11; Myosin-7 Chain: B
Molecule details ›
Chain: B
Length: 973 amino acids
Theoretical weight: 112.05 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10587 (Residues: 2-852; Coverage: 43%)
  • Canonical: P12883 (Residues: 842-963; Coverage: 6%)
Gene names: MYH11, MYH7, MYHCB
Sequence domains:
Myosin light polypeptide 6 Chains: C, D
Molecule details ›
Chains: C, D
Length: 150 amino acids
Theoretical weight: 16.87 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P02607 (Residues: 2-151; Coverage: 99%)
Gene name: MYL6
EF-hand domain-containing protein Chains: E, F
Molecule details ›
Chains: E, F
Length: 196 amino acids
Theoretical weight: 21.71 KDa
Source organism: Avicularia avicularia
UniProt:
  • Canonical: B4XT43 (Residues: 1-196; Coverage: 100%)
Gene name: RLC
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 20Å
Relevant EMDB volumes: EMD-1950
Expression system: Spodoptera frugiperda

Quick links

Citations

22 review citations

Understanding cardiomyopathy phenotypes based on the functional impact of mutations in the myosin motor.
Moore et al. (2012)
21 more

30 mentions without citation

Hypertrophic cardiomyopathy and the myosin mesa: viewing an old disease in a new light.
Trivedi et al. (2018)
29 more