› 3e1w

X-ray diffraction
2.6Å resolution

H. influenzae beta-carbonic anhydrase, variant D44N in 100 mM sodium bicarbonate

Released:
DOI: 10.2210/pdb3e1w/pdb
PDB entry 3e1w coloured by chain, front view.
PDB entry 3e1w coloured by chain, side view.
PDB entry 3e1w coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Allosteric site variants of Haemophilus influenzae beta-carbonic anhydrase.
Rowlett RS, Tu C, Lee J, Herman AG, Chapnick DA, Shah SH, Gareiss PC
Biochemistry 48 6146-56 (2009)
PMID: 19459702

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155252 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.29 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45148 (Residues: 1-229; Coverage: 100%)
Gene names: HI_1301, can
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P41212
Unit cell:
a: 78.45Å b: 78.45Å c: 182.104Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.239
Expression system: Escherichia coli

Quick links

Citations

1 review citation

An Overview of the Bacterial Carbonic Anhydrases.
Supuran et al. (2017)
11 other articles

1 mention without citation

Allosteric site variants of Haemophilus influenzae beta-carbonic anhydrase.
Rowlett et al. (2009)