› 3e3f

X-ray diffraction
2.3Å resolution

H. influenzae beta-carbonic anhydrase, variant V47A with 100 mM bicarbonate

Released:
DOI: 10.2210/pdb3e3f/pdb
PDB entry 3e3f coloured by chain, front view.
PDB entry 3e3f coloured by chain, side view.
PDB entry 3e3f coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Evidence for a bicarbonate "escort" site in Haemophilus influenzae beta-carbonic anhydrase .
Rowlett RS, Hoffmann KM, Failing H, Mysliwiec MM, Samardzic D
Biochemistry 49 3640-7 (2010)
PMID: 20359198

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.26 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45148 (Residues: 1-229; Coverage: 100%)
Gene names: HI_1301, can
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand BCT 5 x BCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P41212
Unit cell:
a: 83.897Å b: 83.897Å c: 184.867Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.23
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Characterization of the first beta-class carbonic anhydrase from an arthropod (Drosophila melanogaster) and phylogenetic analysis of beta-class carbonic anhydrases in invertebrates.
Syrjänen et al. (2010)
3 more

1 mention without citation

Evidence for a bicarbonate "escort" site in Haemophilus influenzae beta-carbonic anhydrase .
Rowlett et al. (2010)