› 3e3q

X-ray diffraction
2.95Å resolution

Structure of the 3alpham13 high-affinity mutant of the 2C TCR in complex with Ld/QL9

Released:
DOI: 10.2210/pdb3e3q/pdb
PDB entry 3e3q coloured by chain, front view.
PDB entry 3e3q coloured by chain, side view.
PDB entry 3e3q coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Distinct CDR3 conformations in TCRs determine the level of cross-reactivity for diverse antigens, but not the docking orientation.
Jones LL, Colf LA, Stone JD, Garcia KC, Kranz DM
J Immunol 181 6255-64 (2008)
PMID: 18941216

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-208733 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class I histocompatibility antigen, L-D alpha chain Chains: A, B, H, L, P, U, Y, c
Molecule details ›
Chains: A, B, H, L, P, U, Y, c
Length: 175 amino acids
Theoretical weight: 20.54 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01897 (Residues: 25-199; Coverage: 52%)
Gene name: H2-L
Sequence domains: Class I Histocompatibility antigen, domains alpha 1 and 2
Structure domains: MHC class I-like antigen recognition-like
QL9 peptide Chains: G, K, O, Q, T, X, b, f
Molecule details ›
Chains: G, K, O, Q, T, X, b, f
Length: 9 amino acids
Theoretical weight: 1.06 KDa
Ig-like domain-containing protein Chains: C, D, I, M, R, V, Z, d
Molecule details ›
Chains: C, D, I, M, R, V, Z, d
Length: 109 amino acids
Theoretical weight: 12.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5R1C2 (Residues: 22-113; Coverage: 99%)
Gene name: TRAV9-4
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
TCR beta chain Chains: E, F, J, N, S, W, a, e
Molecule details ›
Chains: E, F, J, N, S, W, a, e
Length: 111 amino acids
Theoretical weight: 12.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C222
Unit cell:
a: 158.472Å b: 160.458Å c: 357.159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.275
Expression system: Escherichia coli

Quick links

Citations

7 review citations

T-cell receptor binding affinities and kinetics: impact on T-cell activity and specificity.
Stone et al. (2009)
6 more

5 mentions without citation

Distinct CDR3 conformations in TCRs determine the level of cross-reactivity for diverse antigens, but not the docking orientation.
Jones et al. (2008)
4 more