› 3e3t

X-ray diffraction
1.6Å resolution

Structure of porcine pancreatic elastase with the magic triangle I3C

Released:
DOI: 10.2210/pdb3e3t/pdb
PDB entry 3e3t coloured by chain, front view.
PDB entry 3e3t coloured by chain, side view.
PDB entry 3e3t coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
A magic triangle for experimental phasing of macromolecules.
Beck T, Krasauskas A, Gruene T, Sheldrick GM
Acta Crystallogr D Biol Crystallogr 64 1179-82 (2008)
PMID: 19020357

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chymotrypsin-like elastase family member 1 Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 25.93 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00772 (Residues: 27-266; Coverage: 96%)
Gene names: CELA1, ELA1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

4 bound ligands:
Ligand SO4 2 x SO4
Ligand NA 1 x NA
Ligand IOD 1 x IOD
Ligand I3C 4 x I3C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P212121
Unit cell:
a: 50.096Å b: 57.973Å c: 74.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.213

Quick links

Citations

2 review citations

SAD phasing: History, current impact and future opportunities.
Rose et al. (2016)
1 more