› 3e61

X-ray diffraction
2Å resolution

Crystal structure of a putative transcriptional repressor of ribose operon from Staphylococcus saprophyticus subsp. saprophyticus

Released:
DOI: 10.2210/pdb3e61/pdb
PDB entry 3e61 coloured by chain, front view.
PDB entry 3e61 coloured by chain, side view.
PDB entry 3e61 coloured by chain, top view.
Source organism: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292
Entry authors: Bonanno JB, Freeman J, Bain KT, Chang S, Romero R, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lacI-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 277 amino acids
Theoretical weight: 31.38 KDa
Source organism: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q49XF6 (Residues: 54-320; Coverage: 83%)
Gene name: SSP1396
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 81.146Å b: 106.634Å c: 68.183Å
α: 90° β: 112.17° γ: 90°
R-values:
R R work R free
0.214 0.212 0.259
Expression system: Escherichia coli BL21(DE3)

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