› 3ecc

X-ray diffraction
2.7Å resolution

Crystal structure of the DnaC helicase loader in complex with ADP-BeF3

Released:
DOI: 10.2210/pdb3ecc/pdb
PDB entry 3ecc coloured by chain, front view.
PDB entry 3ecc coloured by chain, side view.
PDB entry 3ecc coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structural synergy and molecular crosstalk between bacterial helicase loaders and replication initiators.
Mott ML, Erzberger JP, Coons MM, Berger JM
Cell 135 623-34 (2008)
PMID: 19013274

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AAA+ ATPase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 21.25 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67056 (Residues: 43-226; Coverage: 78%)
Gene names: aq_910, dnaC
Sequence domains: IstB-like ATP binding protein
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand BEF 1 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P61
Unit cell:
a: 84.582Å b: 84.582Å c: 49.587Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.263 0.263 0.294
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Eukaryotic DNA replication control: lock and load, then fire.
Remus et al. (2009)
29 more

7 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
6 more