› 3eff

X-ray diffraction
3.8Å resolution

The Crystal Structure of Full-Length KcsA in its Closed Conformation

Released:
DOI: 10.2210/pdb3eff/pdb
PDB entry 3eff coloured by chain, front view.
PDB entry 3eff coloured by chain, side view.
PDB entry 3eff coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of full-length KcsA in its closed conformation.
Uysal S, Vásquez V, Tereshko V, Esaki K, Fellouse FA, Sidhu SS, Koide S, Perozo E, Kossiakoff A
Proc Natl Acad Sci U S A 106 6644-9 (2009)
PMID: 19346472

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB Chains: A, C
Molecule details ›
Chains: A, C
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
FAB Chains: B, D
Molecule details ›
Chains: B, D
Length: 224 amino acids
Theoretical weight: 23.91 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chains: K, L, M, N
Molecule details ›
Chains: K, L, M, N
Length: 139 amino acids
Theoretical weight: 15.46 KDa
Source organism: Streptomyces lividans
UniProt:
  • Canonical: P0A334 (Residues: 22-160; Coverage: 87%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 115.799Å b: 173.905Å c: 339.425Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.27 0.331

Quick links

Citations

20 review citations

Engineering of recombinant crystallization chaperones.
Koide S. (2009)
19 more

44 mentions without citation

Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes.
Lundbaek et al. (2010)
43 more