› 3eg1

X-ray diffraction
1.85Å resolution

Crystal structure of the N114Q mutant of ABL-SH3 domain complexed with a designed high-affinity peptide ligand: implications for SH3-ligand interactions

Released:
DOI: 10.2210/pdb3eg1/pdb
PDB entry 3eg1 coloured by chain, front view.
PDB entry 3eg1 coloured by chain, side view.
PDB entry 3eg1 coloured by chain, top view.
Source organisms:
Primary publication:
Role of interfacial water molecules in proline-rich ligand recognition by the Src homology 3 domain of Abl.
Palencia A, Camara-Artigas A, Pisabarro MT, Martinez JC, Luque I
J Biol Chem 285 2823-33 (2010)
PMID: 19906645

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132610 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ABL1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00519 (Residues: 60-121; Coverage: 6%)
Gene names: ABL, ABL1, JTK7
Sequence domains: SH3 domain
Structure domains: SH3 Domains
p41 peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.04 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 45.996Å b: 47.636Å c: 55.662Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.185 0.248
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Applications of isothermal titration calorimetry in pure and applied research--survey of the literature from 2010.
Ghai et al. (2012)
2 more

3 mentions without citation

Structural insights into binding of STAC proteins to voltage-gated calcium channels.
Wong King Yuen et al. (2017)
2 more