› 3eng

X-ray diffraction
1.9Å resolution

STRUCTURE OF ENDOGLUCANASE V CELLOBIOSE COMPLEX

Released:
DOI: 10.2210/pdb3eng/pdb
PDB entry 3eng coloured by chain, front view.
PDB entry 3eng coloured by chain, side view.
PDB entry 3eng coloured by chain, top view.
Source organism: Humicola insolens
Primary publication:
Structure determination and refinement of the Humicola insolens endoglucanase V at 1.5 A resolution.
Davies GJ, Dodson G, Moore MH, Tolley SP, Dauter Z, Wilson KS, Rasmussen G, Schülein M
Acta Crystallogr D Biol Crystallogr 52 7-17 (1996)
PMID: 15299721

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155026 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase-5 Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 22.88 KDa
Source organism: Humicola insolens
UniProt:
  • Canonical: P43316 (Residues: 1-213; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 45
Structure domains: RlpA-like domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 41.75Å b: 56.18Å c: 37.37Å
α: 90° β: 93.4° γ: 90°
R-values:
R R work R free
0.145 0.145 not available

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Citations

1 review citation

Understanding the structure and function of bacterial expansins: a prerequisite towards practical applications for the bioenergy and agricultural industries.
Martinez-Anaya C. (2016)
13 other articles

3 mentions without citation

Coarse-grained description of protein internal dynamics: an optimal strategy for decomposing proteins in rigid subunits.
Potestio et al. (2009)
2 more