› 3eo1

X-ray diffraction
3.1Å resolution

Structure of the Fab Fragment of GC-1008 in Complex with Transforming Growth Factor-Beta 3

Released:
DOI: 10.2210/pdb3eo1/pdb
PDB entry 3eo1 coloured by chain, front view.
PDB entry 3eo1 coloured by chain, side view.
PDB entry 3eo1 coloured by chain, top view.
Source organisms:
Primary publication:
A cytokine-neutralizing antibody as a structural mimetic of 2 receptor interactions.
Grütter C, Wilkinson T, Turner R, Podichetty S, Finch D, McCourt M, Loning S, Jermutus L, Grütter MG
Proc Natl Acad Sci U S A 105 20251-6 (2008)
PMID: 19073914

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233903 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GC-1008 Fab Light Chain Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 215 amino acids
Theoretical weight: 23.43 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 4 Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 225 amino acids
Theoretical weight: 23.84 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: P01861 (Residues: 1-105; Coverage: 27%)
Gene name: IGHG4
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Transforming growth factor beta-3 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 112 amino acids
Theoretical weight: 12.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10600 (Residues: 301-412; Coverage: 29%)
Gene name: TGFB3
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 149.04Å b: 149.13Å c: 149.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.279
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

13 review citations

Monoclonal antibodies: versatile platforms for cancer immunotherapy.
Weiner et al. (2010)
12 more

7 mentions without citation

Updates to the Integrated Protein-Protein Interaction Benchmarks: Docking Benchmark Version 5 and Affinity Benchmark Version 2.
Vreven et al. (2015)
6 more