› 3eol

X-ray diffraction
2Å resolution

2.0A crystal structure of isocitrate lyase from Brucella melitensis (P43212)

Released:
DOI: 10.2210/pdb3eol/pdb
PDB entry 3eol coloured by chain, front view.
PDB entry 3eol coloured by chain, side view.
PDB entry 3eol coloured by chain, top view.
Source organism: Brucella melitensis
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
Isocitrate = succinate + glyoxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 433 amino acids
Theoretical weight: 47.21 KDa
Source organism: Brucella melitensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YQA0 (Residues: 1-429; Coverage: 100%)
Gene name: BAB1_1631
Sequence domains: Isocitrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 79.702Å b: 79.702Å c: 281.595Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.263
Expression system: Escherichia coli

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