› 3eqa

X-ray diffraction
1.9Å resolution

Catalytic domain of glucoamylase from aspergillus niger complexed with tris and glycerol

Released:
DOI: 10.2210/pdb3eqa/pdb
PDB entry 3eqa coloured by chain, front view.
PDB entry 3eqa coloured by chain, side view.
PDB entry 3eqa coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Structure of the catalytic domain of glucoamylase from Aspergillus niger.
Lee J, Paetzel M
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 188-92 (2011)
PMID: 21301084

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glucoamylase Chain: A
Molecule details ›
Chain: A
Length: 470 amino acids
Theoretical weight: 50.5 KDa
Source organism: Aspergillus niger
UniProt:
  • Canonical: P69328 (Residues: 25-494; Coverage: 76%)
Gene name: GLAA
Sequence domains: Glycosyl hydrolases family 15
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand MAN 7 x MAN
Ligand TRS 1 x TRS
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 57.826Å b: 73.222Å c: 106.793Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.229

Quick links

Citations

1 review citation

Carbohydrate Binding Modules: Diversity of Domain Architecture in Amylases and Cellulases From Filamentous Microorganisms.
Sidar et al. (2020)
8 other articles

2 mentions without citation

Structure of the catalytic domain of glucoamylase from Aspergillus niger.
Lee et al. (2011)
1 more