› 3ere

X-ray diffraction
2.5Å resolution

Crystal structure of the arginine repressor protein from Mycobacterium tuberculosis in complex with the DNA operator

Released:
DOI: 10.2210/pdb3ere/pdb
PDB entry 3ere coloured by chain, front view.
PDB entry 3ere coloured by chain, side view.
PDB entry 3ere coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure of the arginine repressor protein in complex with the DNA operator from Mycobacterium tuberculosis.
Cherney LT, Cherney MM, Garen CR, Lu GJ, James MN
J Mol Biol 384 1330-40 (2008)
PMID: 18952097

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116214 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Arginine repressor Chain: D
Molecule details ›
Chain: D
Length: 170 amino acids
Theoretical weight: 17.37 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPY9 (Residues: 1-170; Coverage: 100%)
Gene names: MTCY06H11.22, Rv1657, ahrC, argR
Sequence domains:
Structure domains:
5'-D(*DTP*DTP*DGP*DCP*DAP*DTP*DAP*DAP*DCP*DGP*DAP*DTP*DGP*DCP*DAP*DA)-3' Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
5'-D(*DTP*DTP*DGP*DCP*DAP*DTP*DCP*DGP*DTP*DTP*DAP*DTP*DGP*DCP*DAP*DA)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6322
Unit cell:
a: 94.128Å b: 94.128Å c: 147.014Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

The TetR family of regulators.
Cuthbertson et al. (2013)
8 other articles

5 mentions without citation

Structural and Molecular Mechanisms of Cytokine-Mediated Endocrine Resistance in Human Breast Cancer Cells.
Stender et al. (2017)
4 more