› 3f2o

X-ray diffraction
2.05Å resolution

Crystal Structure of human splA/ryanodine receptor domain and SOCS box containing 1 (SPSB1) in complex with a 20-residue VASA peptide

Released:
DOI: 10.2210/pdb3f2o/pdb
PDB entry 3f2o coloured by chain, front view.
PDB entry 3f2o coloured by chain, side view.
PDB entry 3f2o coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for Par-4 recognition by the SPRY domain- and SOCS box-containing proteins SPSB1, SPSB2, and SPSB4.
Filippakopoulos P, Low A, Sharpe TD, Uppenberg J, Yao S, Kuang Z, Savitsky P, Lewis RS, Nicholson SE, Norton RS, Bullock AN
J Mol Biol 401 389-402 (2010)
PMID: 20561531

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140541 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SPRY domain-containing SOCS box protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96BD6 (Residues: 24-233; Coverage: 77%)
Gene names: SPSB1, SSB1
Sequence domains: SPRY domain
ATP-dependent RNA helicase vasa Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 amino acids
Theoretical weight: 2.5 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: P09052 (Residues: 184-203; Coverage: 3%)
Gene names: CG46283, vas, vasa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 75.665Å b: 80.654Å c: 86.864Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.232
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Applications of isothermal titration calorimetry in pure and applied research--survey of the literature from 2010.
Ghai et al. (2012)
3 more

3 mentions without citation

Structural insight into HIV-1 capsid recognition by rhesus TRIM5α.
Yang et al. (2012)
2 more