› 3f3k

X-ray diffraction
1.75Å resolution

The structure of uncharacterized protein YKR043C from Saccharomyces cerevisiae.

Released:
DOI: 10.2210/pdb3f3k/pdb
PDB entry 3f3k coloured by chain, front view.
PDB entry 3f3k coloured by chain, side view.
PDB entry 3f3k coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and activity of the metal-independent fructose-1,6-bisphosphatase YK23 from Saccharomyces cerevisiae.
Kuznetsova E, Xu L, Singer A, Brown G, Dong A, Flick R, Cui H, Cuff M, Joachimiak A, Savchenko A, Yakunin AF
J Biol Chem 285 21049-59 (2010)
PMID: 20427268

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 1,7-bisphosphate + H(2)O = sedoheptulose 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153131 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sedoheptulose 1,7-bisphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 30.51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P36136 (Residues: 2-263; Coverage: 97%)
Gene names: SHB17, YKR043C
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 84.037Å b: 86.277Å c: 100.168Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.141 0.17
Expression system: Escherichia coli

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Citations

12 citation in other articles

Riboneogenesis in yeast.
Clasquin et al. (2011)
11 more

3 mentions without citation

In situ proteolysis to generate crystals for structure determination: an update.
Wernimont et al. (2009)
2 more