› 3f3m

X-ray diffraction
2.4Å resolution

Six Crystal Structures of Two Phosphopantetheine Adenylyltransferases Reveal an Alternative Ligand Binding Mode and an Associated Structural Change

Released:
DOI: 10.2210/pdb3f3m/pdb
PDB entry 3f3m coloured by chain, front view.
PDB entry 3f3m coloured by chain, side view.
PDB entry 3f3m coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
The structure of Staphylococcus aureus phosphopantetheine adenylyltransferase in complex with 3'-phosphoadenosine 5'-phosphosulfate reveals a new ligand-binding mode.
Lee HH, Yoon HJ, Kang JY, Park JH, Kim DJ, Choi KH, Lee SK, Song J, Kim HJ, Suh SW
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 987-91 (2009)
PMID: 19851003

Function and Biology Details

Reaction catalysed: 
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159123 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopantetheine adenylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.47 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63820 (Residues: 1-160; Coverage: 100%)
Gene names: MW1007, coaD
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand PPS 1 x PPS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P6322
Unit cell:
a: 66.774Å b: 66.774Å c: 124.134Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.25
Expression system: Escherichia coli

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Citations

5 mentions without citation

The coenzyme A disulphide reductase of Borrelia burgdorferi is important for rapid growth throughout the enzootic cycle and essential for infection of the mammalian host.
Eggers et al. (2011)
4 more