› 3f5u

X-ray diffraction
2Å resolution

Crystal structure of the death associated protein kinase in complex with AMPPNP and Mg2+

Released:
DOI: 10.2210/pdb3f5u/pdb
PDB entry 3f5u coloured by chain, front view.
PDB entry 3f5u coloured by chain, side view.
PDB entry 3f5u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into nucleotide recognition by human death-associated protein kinase.
McNamara LK, Watterson DM, Brunzelle JS
Acta Crystallogr D Biol Crystallogr 65 241-8 (2009)
PMID: 19237746

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 33.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53355 (Residues: 1-285; Coverage: 20%)
Gene names: DAPK, DAPK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 46.669Å b: 62.461Å c: 88.599Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.245
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Death Associated Protein Kinase 1 (DAPK1): A Regulator of Apoptosis and Autophagy.
Singh et al. (2016)
5 other articles

3 mentions without citation

Structural insight into nucleotide recognition by human death-associated protein kinase.
McNamara et al. (2009)
2 more