› 3f69

X-ray diffraction
2.8Å resolution

Crystal structure of the Mycobacterium tuberculosis PknB mutant kinase domain in complex with KT5720

Released:
DOI: 10.2210/pdb3f69/pdb
PDB entry 3f69 coloured by chain, front view.
PDB entry 3f69 coloured by chain, side view.
PDB entry 3f69 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Auto-activation mechanism of the Mycobacterium tuberculosis PknB receptor Ser/Thr kinase.
Mieczkowski C, Iavarone AT, Alber T
EMBO J 27 3186-97 (2008)
PMID: 19008858

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknB Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 33.55 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WI81 (Residues: 1-308; Coverage: 49%)
Gene names: MTCY10H4.14c, Rv0014c, pknB
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand XDR 2 x XDR
Ligand SO4 4 x SO4
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: F4132
Unit cell:
a: 297.557Å b: 297.557Å c: 297.557Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.268
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Role of eukaryotic-like serine/threonine kinases in bacterial cell division and morphogenesis.
Manuse et al. (2016)
6 more

9 mentions without citation

Eukaryote-like serine/threonine kinases and phosphatases in bacteria.
Pereira et al. (2011)
8 more