› 3f71

X-ray diffraction
1.2Å resolution

Crystal structure of E18D DJ-1 with oxidized C106

Released:
DOI: 10.2210/pdb3f71/pdb
PDB entry 3f71 coloured by chain, front view.
PDB entry 3f71 coloured by chain, side view.
PDB entry 3f71 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Formation of a stabilized cysteine sulfinic acid is critical for the mitochondrial function of the parkinsonism protein DJ-1.
Blackinton J, Lakshminarasimhan M, Thomas KJ, Ahmad R, Greggio E, Raza AS, Cookson MR, Wilson MA
J Biol Chem 284 6476-85 (2009)
PMID: 19124468

Function and Biology Details

Reaction catalysed: 
An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + lactate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parkinson disease protein 7 Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 20.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3121
Unit cell:
a: 74.75Å b: 74.75Å c: 74.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.122 0.122 0.155
Expression system: Escherichia coli

Quick links

Citations

66 review citations

The role of oxidative stress in Parkinson's disease.
Dias et al. (2013)
65 more

1 mention without citation

Formation of a stabilized cysteine sulfinic acid is critical for the mitochondrial function of the parkinsonism protein DJ-1.
Blackinton et al. (2009)