› 3f96

X-ray diffraction
2.1Å resolution

Crystal structure of human plasma platelet activating factor acetylhydrolase covalently inhibited by sarin

Released:
DOI: 10.2210/pdb3f96/pdb
PDB entry 3f96 coloured by chain, front view.
PDB entry 3f96 coloured by chain, side view.
PDB entry 3f96 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human group-VIIA phospholipase A2 inhibited by organophosphorus nerve agents exhibit non-aged complexes.
Samanta U, Kirby SD, Srinivasan P, Cerasoli DM, Bahnson BJ
Biochem Pharmacol 78 420-9 (2009)
PMID: 19394314

Function and Biology Details

Reaction catalysed: 
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Platelet-activating factor acetylhydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 43.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13093 (Residues: 47-429; Coverage: 91%)
Gene names: PAFAH, PLA2G7
Sequence domains: Platelet-activating factor acetylhydrolase, isoform II
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SGB 2 x SGB

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 116.078Å b: 82.455Å c: 96.586Å
α: 90° β: 115.11° γ: 90°
R-values:
R R work R free
0.21 0.206 0.278
Expression system: Escherichia coli

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Citations

4 review citations

Lipoprotein-associated phospholipase A2: The story continues.
Huang et al. (2020)
3 more

4 mentions without citation

Crystal structures of human group-VIIA phospholipase A2 inhibited by organophosphorus nerve agents exhibit non-aged complexes.
Samanta et al. (2009)
3 more