› 3fca

X-ray diffraction
2.15Å resolution

Genetic Incorporation of a Metal-ion Chelating Amino Acid into proteins as biophysical probe

Released:
DOI: 10.2210/pdb3fca/pdb
PDB entry 3fca coloured by chain, front view.
PDB entry 3fca coloured by chain, side view.
PDB entry 3fca coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Genetic incorporation of a metal-ion chelating amino acid into proteins as a biophysical probe.
Lee HS, Spraggon G, Schultz PG, Wang F
J Am Chem Soc 131 2481-3 (2009)
PMID: 19193005

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 31.24 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand HQA 2 x HQA

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P42212
Unit cell:
a: 135.858Å b: 135.858Å c: 74.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.244
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Adding new chemistries to the genetic code.
Liu et al. (2010)
25 more