› 3fdd

X-ray diffraction
2.35Å resolution

The Crystal Structure of the Pseudomonas dacunhae Aspartate-Beta-Decarboxylase Reveals a Novel Oligomeric Assembly for a Pyridoxal-5-Phosphate Dependent Enzyme

Released:
DOI: 10.2210/pdb3fdd/pdb
PDB entry 3fdd coloured by chain, front view.
PDB entry 3fdd coloured by chain, side view.
PDB entry 3fdd coloured by chain, top view.
Source organism: [Pseudomonas] dacunhae ATCC 21192
Primary publication:
The crystal structure of the Pseudomonas dacunhae aspartate-beta-decarboxylase dodecamer reveals an unknown oligomeric assembly for a pyridoxal-5'-phosphate-dependent enzyme.
Lima S, Sundararaju B, Huang C, Khristoforov R, Momany C, Phillips RS
J Mol Biol 388 98-108 (2009)
PMID: 19265705

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183005 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 533 amino acids
Theoretical weight: 59.58 KDa
Source organism: [Pseudomonas] dacunhae ATCC 21192
Expression system: Escherichia coli
UniProt:
  • Canonical: Q845W8 (Residues: 1-533; Coverage: 100%)
Gene name: aspD
Sequence domains: Aminotransferase class I and II
Structure domains: Aspartate Aminotransferase, domain 1

Ligands and Environments

3 bound ligands:
Ligand PLP 1 x PLP
Ligand ACT 1 x ACT
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I23
Unit cell:
a: 150.453Å b: 150.453Å c: 150.453Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.212
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Controlling reaction specificity in pyridoxal phosphate enzymes.
Toney MD. (2011)
3 other articles

2 mentions without citation

Lessons from high-throughput protein crystallization screening: 10 years of practical experience.
Luft et al. (2011)
1 more