› 3fl0

X-ray diffraction
1.94Å resolution

X-ray structure of the non covalent swapped form of the Q28L/K31C/S32C mutant of bovine pancreatic ribonuclease in complex with 2'-DEOXYCYTIDINE-2'-DEOXYGUANOSINE-3',5'-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb3fl0/pdb
PDB entry 3fl0 coloured by chain, front view.
PDB entry 3fl0 coloured by chain, side view.
PDB entry 3fl0 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Toward an antitumor form of bovine pancreatic ribonuclease: the crystal structure of three noncovalent dimeric mutants.
Merlino A, Russo Krauss I, Perillo M, Mattia CA, Ercole C, Picone D, Vergara A, Sica F
Biopolymers 91 1029-37 (2009)
PMID: 19280639

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.8 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand CGP 1 x CGP
1 modified residue:
Ligand YCM 4 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 37.09Å b: 62.35Å c: 56.64Å
α: 90° β: 93.63° γ: 90°
R-values:
R R work R free
0.188 0.174 0.227
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Ribonucleases of different origins with a wide spectrum of medicinal applications.
Fang et al. (2011)
1 more