› 3fpl

X-ray diffraction
1.9Å resolution

Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of C. beijerinckii ADH by T. brockii ADH

Released:
DOI: 10.2210/pdb3fpl/pdb
PDB entry 3fpl coloured by chain, front view.
PDB entry 3fpl coloured by chain, side view.
PDB entry 3fpl coloured by chain, top view.
Source organisms:
Primary publication:
Biochemical and structural properties of chimeras constructed by exchange of cofactor-binding domains in alcohol dehydrogenases from thermophilic and mesophilic microorganisms.
Goihberg E, Peretz M, Tel-Or S, Dym O, Shimon L, Frolow F, Burstein Y
Biochemistry 49 1943-53 (2010)
PMID: 20102159

Function and Biology Details

Reaction catalysed: 
Propan-2-ol + NADP(+) = acetone + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent isopropanol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 37.65 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P14941 (Residues: 1-7, 165-301; Coverage: 41%)
  • Canonical: P25984 (Residues: 8-164, 302-351; Coverage: 59%)
Gene name: adh
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 2 x CL
Ligand EDO 1 x EDO
Ligand CAC 1 x CAC
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: I23
Unit cell:
a: 129.477Å b: 129.477Å c: 129.477Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.122 0.172
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains.
Collas et al. (2012)
1 more