› 3fpu

X-ray diffraction
1.76Å resolution

The crystallographic structure of the Complex between Evasin-1 and CCL3

Released:
DOI: 10.2210/pdb3fpu/pdb
PDB entry 3fpu coloured by chain, front view.
PDB entry 3fpu coloured by chain, side view.
PDB entry 3fpu coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of chemokine sequestration by a tick chemokine binding protein: the crystal structure of the complex between Evasin-1 and CCL3.
Dias JM, Losberger C, Déruaz M, Power CA, Proudfoot AE, Shaw JP
PLoS One 4 e8514 (2009)
PMID: 20041127

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143174 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Evasin-1 Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.31 KDa
Source organism: Rhipicephalus sanguineus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0C8E7 (Residues: 21-114; Coverage: 100%)
Sequence domains: Evasins Class A
Structure domains: Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4
C-C motif chemokine 3 Chain: B
Molecule details ›
Chain: B
Length: 70 amino acids
Theoretical weight: 7.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10147 (Residues: 24-92; Coverage: 100%)
Gene names: CCL3, G0S19-1, MIP1A, SCYA3
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P4332
Unit cell:
a: 104.384Å b: 104.384Å c: 104.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.285
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

8 review citations

International Union of Basic and Clinical Pharmacology. [corrected]. LXXXIX. Update on the extended family of chemokine receptors and introducing a new nomenclature for atypical chemokine receptors.
Bachelerie et al. (2014)
7 more

14 mentions without citation

Evasins: Tick Salivary Proteins that Inhibit Mammalian Chemokines.
Bhusal et al. (2020)
13 more