› 3fra

X-ray diffraction
2.35Å resolution

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: X
Molecule details ›
Chain: X
Length: 158 amino acids
Theoretical weight: 18.16 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A017 (Residues: 2-159; Coverage: 99%)
Gene name: folA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
Ligand I2H 1 x I2H
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P6122
Unit cell:
a: 79.2Å b: 79.2Å c: 108.531Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.294
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Antibiotic resistance in Staphylococcus aureus. Current status and future prospects.
Foster TJ. (2017)
14 more

4 mentions without citation

Antifolate agents: a patent review (2006 - 2010).
Wright et al. (2011)
3 more