› 3frl

X-ray diffraction
2.25Å resolution

The 2.25 A crystal structure of LipL32, the major surface antigen of Leptospira interrogans serovar Copenhageni

Released:
DOI: 10.2210/pdb3frl/pdb
PDB entry 3frl coloured by chain, front view.
PDB entry 3frl coloured by chain, side view.
PDB entry 3frl coloured by chain, top view.
Source organism: Leptospira interrogans serovar Copenhageni
Primary publication:
Structure and calcium-binding activity of LipL32, the major surface antigen of pathogenic Leptospira sp.
Hauk P, Guzzo CR, Roman Ramos H, Ho PL, Farah CS
J Mol Biol 390 722-36 (2009)
PMID: 19477185

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Surface lipoprotein of Spirochaetales order domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 27.82 KDa
Source organism: Leptospira interrogans serovar Copenhageni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72SM7 (Residues: 21-272; Coverage: 100%)
Gene names: LIC_11352, lipL32
Sequence domains: Surface lipoprotein of Spirochaetales order

Ligands and Environments

3 bound ligands:
Ligand 211 1 x 211
Ligand OXL 2 x OXL
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P3221
Unit cell:
a: 126.725Å b: 126.725Å c: 95.996Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.181 0.227
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The lipoprotein LipL32, an enigma of leptospiral biology.
Murray GL. (2013)
3 more

3 mentions without citation

Essential calcium-binding cluster of Leptospira LipL32 protein for inflammatory responses through the Toll-like receptor 2 pathway.
Lo et al. (2013)
2 more